Steve Clarke says Nathan Patterson is "uncertain" to face Ukraine on 1 June - however is optimistic the right-back will function at some stage subsequent month.
Patterson made the lower regardless of fears over his health and joins fellow harm doubt Billy Gilmour within the squad.
Kieran Tierney misses out with harm and Clarke confirmed he has identified for "weeks" he would't make it.
Scotland face Ukraine earlier than a possible playoff ultimate with Wales on 5 June.
Clarke's crew then have a triple header of Nations League video games with two clashes with Armenia both aspect of the journey to face Eire.
On the harm scenario Clarke mentioned: "It is a massive blow for everyone simply discovering out at this time however we have identified for a short time Kieran would not be concerned.
"It is disappointing for Kieran and for us.
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"At some stage over the 4 or hopefully 5 matches, Nathan shall be match to play. His return to play is imminent and it'll simply come all the way down to match health.
"I'd put him down as uncertain for the Ukraine sport.
"Billy shall be high quality. Clearly disappointing for somebody like Kenny McLean who has missed out once more after selecting up a damaged toe.
"That is disappointing as a result of he is a vital a part of the squad."
The supervisor additionally dismissed speak Scotland would be the "unhealthy guys" as they get set to tackle Ukraine after the preliminary November date was postponed as a result of harrowing battle with Russia.
"I do not assume it is the primary time we have been the unhealthy guys," he mentioned. "All of us perceive and respect the place of the Ukrainian crew.
"Thankfully for them they've managed to get their crew in another country and right into a coaching camp. They have been enjoying matches and they are going to be prepared for the sport.
"We'll be prepared too. It may be two groups who're able to go."