Ayr lastly discovered the way in which to aim at Ochilview as a grandstand comeback received three factors towards Queen's Park.
United had been compelled to return from 2-0 down after a tardy first half left them with a mountain to climb.
However Dipo Akinyemi's first aim for the membership, adopted by a captain's header from Sean McGinty, arrange an enormous end as Akinyemi then buried a stoppage-time penalty to win it.
Lee Bullen had caught with the identical workforce that opened the Championship season with a goalless draw towards Arbroath.
However they did not look almost as threatening as final week's model, with Queen's dominating possession within the early phases.
And the Spiders took a deserved lead with the sport 17 minutes previous when a easy set piece proved Ayr's undoing.
Dom Thomas was creator with a nook from the appropriate and Simon Murray had the liberty of Ochilview to climb and head house from six yards.
Ayr continued to be their very own worst enemy and duly fell two behind simply earlier than the half-hour mark.
Queen's had been as soon as once more given far an excessive amount of time and area within the ultimate third and, when the ball ultimately discovered its approach to Thomas, he curled house a superb end from 20 yards.
That lastly woke Ayr from their slumber and Fraser Bryden virtually received them again in it with a low header properly saved by Calum Ferrie.
The younger striker ought to then have scored earlier than the break when clear by means of however he blazed excessive.
Bullen made a double change on the interval with Mark McKenzie an Daire O'Connor changing Bryden and Jayden Mitchell-Lawson.
And the modifications had a right away influence with McKenzie enjoying in Akinyemi to fireside house his first Ayr aim in entrance of the travelling help.
Obtain the Ayrshire Stay app as we speak
The Ayrshire Stay app is out there to obtain now.
Get all of the native information in your space – plus options, soccer information and the newest on the coronavirus disaster – at your fingertips 24/7.
The free obtain options the newest breaking information and unique tales when you can customise your web page with the sections that matter to you.
The Ayrshire Stay app is out there to obtain now on iOS and Android.
United had been virtually caught chilly on the different finish when a Murray shot was creeping within the backside nook however Charlie Albinson received all the way down to maintain Ayr in it.
Ayr had been proving a unique animal in assault, although, with McKenzie and O'Connor including a brand new dimension and McKenzie drew an incredible cease from Ferrie earlier than O'Connor fired inches over as they threw every little thing at a leveller.
Spiders boss Owen Coyle reached for his bench to stem the tide and referred to as on Nigerian worldwide defender Stephen Eze to attempt to shore up their backline.
But it surely did little to vary the sample of play and Ayr lastly compelled the equaliser when McGinty rose to bullet house a header from a Ben Dempsey nook.
And simply because it regarded like Ayr must accept some extent, they received a penalty deep in stoppage-time when Charlie Fox dealt with within the field and Akinyemi stepped as much as ram house and ship the travelling help wild.
Do not miss the newest Ayrshire headlines –signal as much as our free each day e-newsletter right here